Single-Molecule Fluorescence Biophysics
Single-Molecule Fluorescence Microscopy, using biochemical techniques to obtain chemical selectivity, has developed into a very powerful tool in biophysical and biomedical research that provides the ultimate level of sensitivity and avoids ensemble averaging. Our group is focusing on the further development and application of advanced single-molecule spectroscopy techniques that are instrumental in the study of fundamental problems in Biophysics. Current research activities include:
· the analysis and control of conformational dynamics in a membrane transporter protein “nano-machine”,
· the probing of hydrodynamic properties and dynamics of proteins by a novel rotational fluorescence correlation spectroscopy,
· and the study of the photon statistics in the fluorescence from single pigment-protein complexes.
(last modified: 15/03/2011, wm)