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Diamond Materials

Diamond Materials for Quantum Application

23. September 2014: The DFG research group FOR 1493 “Diamond Materials and Quantum Applications” goes into its second funding period. FOR1493 is a national research consortium funded by the Deutsche Forsch-ungsgemeinschaft.

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ERC Advanced Grant

ERC

Subcellular Raman Microspectroscopy Imaging ...

... of Nucleic Acids and Tryptophan for Distinction of Normal Human Skin Cells and Tumorigenic Keratinocytes

Tumor diagnostic imaging is commonly based on staining of biopsies, which requires tissue excision, fixation, and staining with exogenous marker molecules. Here, P. Piredda and A. Volkmer demonstrate label-free tumor imaging using confocal spontaneous Raman scattering microspectroscopy, which exploits the intrinsic vibrational contrast of endogenous biomolecular species. In a collaboration with M. Berning and P. Boukamp of the DKFZ in Heidelberg, we present a chemically specific and quantitative approach to monitoring normal human skin cells as well as the human HaCaT in vitro skin carcinogenesis model and the tumor-derived MET in vivo skin cancer progression model. Mapping the amplitudes of two spectrally well isolated Raman bands allowed for direct visualization of the distributions representative of tryptophan-rich proteins and nucleic acids with subcellular spatial resolution. Using these Raman markers, it was feasible to discriminate between normal human epidermal keratinocytes and dermal fibroblasts, and to confine all tumorigenic cells from both. First evidence for the successful application of the proposed intracellular nucleic acid and tryptophan Raman signatures for skin cancer diagnosis was further demonstrated in an organotypic cutaneous squamous cell carcinomas model, allowing for the identification of tumor cells and their surrounding stroma in the tissue context.

Reference: P. Piredda, M. Berning, P. Boukamp, and A. Volkmer, Subcellular Raman Microspectroscopy Imaging of Nucleic Acids and Tryptophan for Distinction of Normal Human Skin Cells and Tumorigenic Keratinocytes, Anal. Chem. 87 (2015), advance online, DOI: 10.1021/acs.analchem.5b01009

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